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BORGES Frederic

BORGES Frederic

  • Status : Maitre de conférences HDR
  • Email address : frederic.borges@univ-lorraine.fr
  • Speciality : Food Microbial Ecosystem Engineering
  • Phone number : +33 (0)3 83 59 58 83

Activities :

Profiles:

ResearchGate

Citation Google Scholar

  

    I'm a food microbiologist at the University of Lorraine in France. My research focuses on the engineering of fermented food ecosystems. The objective is to design new approaches to engineering microbial cultures for the food industry. The targeted application is mainly biopreservation, a sustainable approach to increase food safety and extend food shelf-life.  My team has developed high-throughput phenotyping approaches to study the behaviour of candidate microorganisms in a multi-dimensional space. The ambition is to take into account the ecosystemic complexity of foods in order to obtain food microbiomes that are resistant to colonisation by undesirable microorganisms with high robustness.

Keywords: Fermented Foods, Cheese, Dairy, Engineering, Microbial ecosystem, Biopreservation, Bacteriocins, High-Throughput Screening, High-Throughput Phenotyping, Genomics, Metagenomics, Lactic Acid Bacteria

 

EDUCATION

1999 - University degree in Biochemistry, Henry Poincaré University, Nancy, France

2001 – M.S. in Biochemistry, Henry Poincaré University, Nancy, France

2005 – PhD in physiology and molecular genetics, Henry Poincaré University, Nancy, France

2019 - Accreditation to Supervise Research (HDR), University of Lorraine, Nancy, France

 

ACADEMIC POSITIONS

  • 2005-2007 - Postdoc on the molecular pathogenesis of Streptococcus agalactiae, Institute of Microbiology and Biotechnology, University of Ulm

- Genetic engineering: heterologous protein expression, mutant construction

- Immunoassays: Western Blots, ELISA

- Bacteria-host interaction: cell line culture, adhesion and invasion assays

  • 2007-2008 - Postdoc on Cheese Microbial Diversity, Food Science and Engineering Laboratory (LSGA), University of Lorraine – ENSAIA

 

  • Microbial diversity : TTGE

Since 2008 : Associate Professor in Food Microbial Engineering, Laboratory of Biomolecules Engineering (LIBio), University of Lorraine – ENSAIA

- Genomics: assembly in Galaxy environment, annotation and comparative genome analysis with MicroScope Mage

- Microbial Diversity: MultiLocus Sequence Typing (MLST), taxonomic identification

- Community structure: metabarcoding in Galaxy and R

- High-Throughput phenotyping: bacterial collection screening, Inhibition graph analysis

- Data analysis: data manipulation and statistical analysis with the R programming langage

 

PATENT

BORGES Frédéric, REVOL-JUNELLES Anne-Marie, NOVEL STRAINS OF CARNOBACTERIUM MALTAROMATICUM AND USES THEREOF. WO2021078612 (A1). 2021-04-29.

 

PUBLICATIONS

  • 2021

George, F., Mahieux, S., Daniel, C., Titécat, M., Beauval, N., Houcke, I., Neut, C., Allorge, D., Borges, F., Jan, G., Foligné, B., Garat, A., 2021. Assessment of Pb(II), Cd(II), and Al(III) Removal Capacity of Bacteria from Food and Gut Ecological Niches: Insights into Biodiversity to Limit Intestinal Biodisponibility of Toxic Metals. Microorganisms 9, 456. https://doi.org/10.3390/microorganisms9020456

Dos Santos Morais, R., Louvet, N., Borges, F., Dumas, D., Cvetkovska-Ben Mohamed, L., Barrau, S., et al. (2021). Impact of Lacticaseibacillus rhamnosus GG on the Emulsion Stability of Raw Milk. Foods 10, 991. https://doi.org/10.3390/foods10050991

Li, H., Ramia, N. E., Borges, F., Revol-Junelles, A.-M., Vogensen, F. K., and Leisner, J. J. (2021). Identification of Potential Citrate Metabolism Pathways in Carnobacterium maltaromaticum. Microorganisms 9, 2169. https://doi.org/10.3390/microorganisms9102169

 

  • 2020

Dos Santos Morais, R., El-Kirat-Chatel, S., Burgain, J., Simard, B., Barrau, S., Paris, C., Borges, F., Gaiani, C., 2020. A Fast, Efficient and Easy to Implement Method to Purify Bacterial Pili From Lacticaseibacillus rhamnosus GG Based on Multimodal Chromatography. Front. Microbiol. 11. https://doi.org/10.3389/fmicb.2020.609880

Gomand, F., H. Mitchell, W., Burgain, J., Petit, J., Borges, F., E. Spagnolie, S., Gaiani, C., 2020. Shaving and breaking bacterial chains with a viscous flow. Soft Matter 16, 9273–9291. https://doi.org/10.1039/D0SM00292E

Nitschel, R., Ankenbauer, A., Welsch, I., Wirth, N.T., Massner, C., Ahmad, N., McColm, S., Borges, F., Fotheringham, I., Takors, R., Blombach, B., 2020. Engineering Pseudomonas putida KT2440 for the production of isobutanol. Eng. Life Sci. 20, 148–159. https://doi.org/10.1002/elsc.201900151

Ramia, N.E., Mangavel, C., Gaiani, C., Muller-Gueudin, A., Taha, S., Revol-Junelles, A.-M., Borges, F., 2020. Nested structure of intraspecific competition network in Carnobacterium maltaromaticum. Sci. Rep. 10, 1–9. https://doi.org/10.1038/s41598-020-63844-5

 

  • 2019

Gomand, F., Borges, F., Burgain, J., Guerin, J., Revol-Junelles, A.-M., Gaiani, C., 2019. Food Matrix Design for Effective Lactic Acid Bacteria Delivery. Annual Review of Food Science and Technology 10, null. https://doi.org/10.1146/annurev-food-032818-121140

Gomand, F., Borges, F., Guerin, J., El-Kirat-Chatel, S., Francius, G., Dumas, D., Burgain, J., Gaiani, C., 2019. Adhesive Interactions Between Lactic Acid Bacteria and β-Lactoglobulin: Specificity and Impact on Bacterial Location in Whey Protein Isolate. Front Microbiol 10, 1512. https://doi.org/10.3389/fmicb.2019.01512

Iskandar, C.F., Cailliez-Grimal, C., Borges, F., Revol-Junelles, A.-M., 2019. Review of lactose and galactose metabolism in Lactic Acid Bacteria dedicated to expert genomic annotation. Trends in Food Science & Technology 88, 121–132. https://doi.org/10.1016/j.tifs.2019.03.020

 

  • 2018

Ramia, N.E., El Kheir, S.M., Taha, S., Mangavel, C., Revol-Junelles, A.M., Borges, F., 2018. Multilocus sequence typing of Carnobacterium maltaromaticum strains associated with fish disease and dairy products. J. Appl. Microbiol. https://doi.org/10.1111/jam.14127

El Kheir, S.M., Cherrat, L., Awussi, A.A., Ramia, N.E., Taha, S., Rahman, A., Passerini, D., Leroi, F., Petit, J., Mangavel, C., Revol-Junelles, A.-M., Borges, F., 2018. High-Throughput Identification of candidate strains for biopreservation by using bioluminescent Listeria monocytogenes. Front. Microbiol. 9. https://doi.org/10.3389/fmicb.2018.01883

George, F., Daniel, C., Thomas, M., Singer, E., Guilbaud, A., Tessier, F.J., Revol-Junelles, A.-M., Borges, F., Foligné, B., 2018. Occurrence and Dynamism of Lactic Acid Bacteria in Distinct Ecological Niches: A Multifaceted Functional Health Perspective. Front. Microbiol. 9. https://doi.org/10.3389/fmicb.2018.02899

Gomand, F., Borges, F., Salim, D., Burgain, J., Guerin, J., Gaiani, C., 2018. High-throughput screening approach to evaluate the adhesive properties of bacteria to milk biomolecules. Food Hydrocolloids 84, 537–544. https://doi.org/10.1016/j.foodhyd.2018.06.038

Guerin, J., Soligot, C., Burgain, J., Huguet, M., Francius, G., El-Kirat-Chatel, S., Gomand, F., Lebeer, S., Le Roux, Y., Borges, F., Scher, J., Gaiani, C., 2018. Adhesive interactions between milk fat globule membrane and Lactobacillus rhamnosus GG inhibit bacterial attachment to Caco-2 TC7 intestinal cell. Colloids and Surfaces B: Biointerfaces 167, 44–53. https://doi.org/10.1016/j.colsurfb.2018.03.044

 

  • 2017

Iskandar, C.F., Borges, F., Taminiau, B., Daube, G., Zagorec, M., Remenant, B., Leisner, J.J., Hansen, M.A., Sørensen, S.J., Mangavel, C., Cailliez-Grimal, C., Revol-Junelles, A.-M., 2017. Comparative Genomic Analysis Reveals Ecological Differentiation in the Genus Carnobacterium. Front. Microbiol. 8. https://doi.org/10.3389/fmicb.2017.00357

Guerin, J., Burgain, J., Borges, F., Bhandari, B., Desobry, S., Scher, J., Gaiani, C., 2017a. Use of imaging techniques to identify efficient controlled release systems of Lactobacillus rhamnosus GG during in vitro digestion. Food Funct. https://doi.org/10.1039/C6FO01737A

Guerin, J., Petit, J., Burgain, J., Borges, F., Bhandari, B., Perroud, C., Desobry, S., Scher, J., Gaiani, C., 2017b. Lactobacillus rhamnosus GG encapsulation by spray-drying: Milk proteins clotting control to produce innovative matrices. Journal of Food Engineering 193, 10–19. https://doi.org/10.1016/j.jfoodeng.2016.08.008

 

  • 2016

Rahman, A., El Kheir, S.M., Back, A., Mangavel, C., Revol-Junelles, A.-M., Borges, F., 2016. Repeat-based Sequence Typing of Carnobacterium maltaromaticum. International Journal of Food Microbiology 226, 1–4. https://doi.org/10.1016/j.ijfoodmicro.2016.03.003

Remenant, B., Borges, F., Cailliez-Grimal, C., Revol-Junelles, A.-M., Marché, L., Lajus, A., Médigue, C., Pilet, M.-F., Prévost, H., Zagorec, M., 2016. Draft Genome Sequence of Carnobacterium divergens V41, a Bacteriocin-Producing Strain. Genome Announc. 4, e01109-16. https://doi.org/10.1128/genomeA.01109-16

Iskandar, C.F., Cailliez-Grimal, C., Rahman, A., Rondags, E., Remenant, B., Zagorec, M., Leisner, J.J., Borges, F., Revol-Junelles, A.-M., 2016. Genes associated to lactose metabolism illustrate the high diversity of Carnobacterium maltaromaticum. Food Microbiology 58, 79–86. https://doi.org/10.1016/j.fm.2016.03.008

Guerin, J., Bacharouche, J., Burgain, J., Lebeer, S., Francius, G., Borges, F., Scher, J., Gaiani, C., 2016. Pili of Lactobacillus rhamnosus GG mediate interaction with β-lactoglobulin. Food Hydrocolloids 58, 35–41. https://doi.org/10.1016/j.foodhyd.2016.02.016

 

  • 2015

Back, A., Borges, F., Mangavel, C., Paris, C., Rondags, E., Kapel, R., Aymes, A., Rogniaux, H., Pavlović, M., van Heel, A.J., Kuipers, O.P., Revol-Junelles, A.-M., Cailliez-Grimal, C., 2015. Recombinant pediocin in Lactococcus lactis: increased production by propeptide fusion and improved potency by co-production with PedC. Microbial Biotechnology n/a-n/a. https://doi.org/10.1111/1751-7915.12285

 

  • 2014

Rahman, A., Gleinser, M., Lanhers, M.-C., Riedel, C.U., Foligné, B., Hanse, M., Yen, F.T., Klouj, A., Afzal, M.I., Back, A., Mangavel, C., Cailliez-Grimal, C., Revol-Junelles, A.-M., Borges, F., 2014b. Adaptation of the lactic acid bacterium Carnobacterium maltaromaticum LMA 28 to the mammalian gastrointestinal tract: From survival in mice to interaction with human cells. International Dairy Journal 34, 93–99. https://doi.org/10.1016/j.idairyj.2013.07.003

Rahman, A., Cailliez-Grimal, C., Bontemps, C., Payot, S., Chaillou, S., Revol-Junelles, A.-M., Borges, F., 2014b. High genetic diversity among strains of the unindustrialized lactic acid bacterium Carnobacterium maltaromaticum in dairy products as revealed by multilocus sequence typing. Appl. Environ. Microbiol. 80, 3920–3929. https://doi.org/10.1128/AEM.00681-14

Burgain, J., Scher, J., Francius, G., Borges, F., Corgneau, M., Revol-Junelles, A.M., Cailliez-Grimal, C., Gaiani, C., 2014. Lactic acid bacteria in dairy food: surface characterization and interactions with food matrix components. Adv Colloid Interface Sci 213, 21–35. https://doi.org/10.1016/j.cis.2014.09.005

 

  • 2013

Cailliez-Grimal, C., Chaillou, S., Anba-Mondoloni, J., Loux, V., Afzal, M.I., Rahman, A., Kergourlay, G., Champomier-Vergès, M.-C., Zagorec, M., Dalgaard, P., Leisner, J.J., Prévost, H., Revol-Junelles, A.-M., Borges, F., 2013. Complete Chromosome Sequence of Carnobacterium maltaromaticum LMA 28. Genome Announc 1. https://doi.org/10.1128/genomeA.00115-12

Afzal, M.I., Gonzalez Ariceaga, C.C., Lhomme, E., Kamel Ali, N., Payot, S., Burgain, J., Gaiani, C., Borges, F., Revol-Junelles, A.M., Delaunay, S., Cailliez-Grimal, C., 2013. Characterization of Carnobacterium maltaromaticum LMA 28 for its positive technological role in soft cheese making. Food Microbiol 36, 223–30.

 

  • 2012

Afzal, M.I., Delaunay, S., Paris, C., Borges, F., Revol-Junelles, A.M., Cailliez-Grimal, C., 2012. Identification of metabolic pathways involved in the biosynthesis of flavor compound 3-methylbutanal from leucine catabolism by Carnobacterium maltaromaticum LMA 28. International journal of food microbiology 157, 332–9.

Jacquet, T, Cailliez-Grimal, C., Borges, F., Gaiani, C., Francius, G., Duval, J.F.L., Waldvogel, Y., Revol-Junelles, A.-M., 2012. Surface properties of bacteria sensitive and resistant to the class IIa carnobacteriocin Cbn BM1. J. Appl. Microbiol. 112, 372–382. https://doi.org/10.1111/j.1365-2672.2011.05195.x

Jacquet, Thibaut, Cailliez-Grimal, C., Francius, G., Borges, F., Imran, M., Duval, J.F.L., Revol-Junelles, A.-M., 2012. Antibacterial activity of class IIa bacteriocin Cbn BM1 depends on the physiological state of the target bacteria. Res. Microbiol. 163, 323–331. https://doi.org/10.1016/j.resmic.2012.04.001

 

  • 2011

Samen, U., Heinz, B., Boisvert, H., Eikmanns, B.J., Reinscheid, D.J., Borges, F., 2011. Rga is a regulator of adherence and pilus formation in Streptococcus agalactiae. Microbiology (Reading, Engl.) 157, 2319–2327. https://doi.org/10.1099/mic.0.044933-0

 

  • 2010

Afzal, M.I., Jacquet, T., Delaunay, S., Borges, F., Millière, J.-B., Revol-Junelles, A.-M., Cailliez-Grimal, C., 2010. Carnobacterium maltaromaticum: identification, isolation tools, ecology and technological aspects in dairy products. Food Microbiol. 27, 573–579. https://doi.org/10.1016/j.fm.2010.03.019

 

  • 2009

Serhan, M., Cailliez-Grimal, C., Borges, F., Revol-Junelles, A.M., Hosri, C., Fanni, J., 2009. Bacterial diversity of Darfiyeh, a Lebanese artisanal raw goat’s milk cheese. Food Microbiol 26, 645–52.

Layec, S., Gérard, J., Legué, V., Chapot-Chartier, M.-P., Courtin, P., Borges, F., Decaris, B., Leblond-Bourget, N., 2009. The CHAP domain of Cse functions as an endopeptidase that acts at mature septa to promote Streptococcus thermophilus cell separation. Mol. Microbiol. 71, 1205–1217. https://doi.org/10.1111/j.1365-2958.2009.06595.x

 

  • 2007

Samen, U., Eikmanns, B.J., Reinscheid, D.J., Borges, F., 2007. The surface protein Srr-1 of Streptococcus agalactiae binds human keratin 4 and promotes adherence to epithelial HEp-2 cells. Infect. Immun. 75, 5405–5414. https://doi.org/10.1128/IAI.00717-07

 

  • 2006

Borges, F., Layec, S., Fernandez, A., Decaris, B., Leblond-Bourget, N., 2006. High genetic variability of the Streptococcus thermophilus cse central part, a repeat rich region required for full cell segregation activity. Antonie Van Leeuwenhoek 90, 245–255. https://doi.org/10.1007/s10482-006-9079-5

Fernandez, A., Borges, F., Gintz, B., Decaris, B., Leblond-Bourget, N., 2006. The rggC locus, with a frameshift mutation, is involved in oxidative stress response by Streptococcus thermophilus. Arch. Microbiol. 186, 161–169. https://doi.org/10.1007/s00203-006-0130-8

Choulet, F., Gallois, A., Aigle, B., Mangenot, S., Gerbaud, C., Truong, C., Francou, F.-X., Borges, F., Fourrier, C., Guérineau, M., Decaris, B., Barbe, V., Pernodet, J.-L., Leblond, P., 2006. Intraspecific Variability of the Terminal Inverted Repeats of the Linear Chromosome of Streptomyces ambofaciens. J. Bacteriol. 188, 6599–6610. https://doi.org/10.1128/JB.00734-06

 

  • 2005

Borges, F., Layec, S., Thibessard, A., Fernandez, A., Gintz, B., Hols, P., Decaris, B., Leblond-Bourget, N., 2005. cse, a Chimeric and variable gene, encodes an extracellular protein involved in cellular segregation in Streptococcus thermophilus. J. Bacteriol. 187, 2737–2746. https://doi.org/10.1128/JB.187.8.2737-2746.2005

 

  • 2004

Thibessard, A., Borges, F., Fernandez, A., Gintz, B., Decaris, B., Leblond-Bourget, N., 2004. Identification of Streptococcus thermophilus CNRZ368 genes involved in defense against superoxide stress. Appl. Environ. Microbiol. 70, 2220–2229.

Fernandez, A., Borges, F., Thibessard, A., Gintz, B., Decaris, B., Leblond-Bourget, N., 2004a. Characterisation of Streptococcus thermophilus CNRZ368 oxidative stress-resistant mutants: involvement of a potential Rgg-like transcriptional regulator. Le Lait 84, 77–85. https://doi.org/10.1051/lait:2003043

Fernandez, A., Thibessard, A., Borges, F., Gintz, B., Decaris, B., Leblond-Bourget, N., 2004b. Characterization of oxidative stress-resistant mutants of Streptococcus thermophilus CNRZ368. Arch. Microbiol. 182, 364–372. https://doi.org/10.1007/s00203-004-0712-2

 

  • 2002

Josse, D., Ebel, C., Stroebel, D., Fontaine, A., Borges, F., Echalier, A., Baud, D., Renault, F., Maire, M. le, Chabrières, E., Masson, P., 2002. Oligomeric States of the Detergent-solubilized Human Serum Paraoxonase (PON1). J. Biol. Chem. 277, 33386–33397. https://scholar.google.be/citations?user=9ieddhkAAAAJ&hl=fr